agr2  (Novus Biologicals)

Journal: BMC Cancer

Article Title: High expression of AGR2 in lung cancer is predictive of poor survival

doi: 10.1186/s12885-015-1658-2

Figure Lengend Snippet: Patient demographics and AGR2 expression by subgroups

Article Snippet: AGR2 expression was evaluated on the TMA with NB110-17780 rabbit polyclonal (Novus Biologicals) at 2 μg/ml concentration as described [ ].

Techniques: Expressing

Journal: BMC Cancer

Article Title: High expression of AGR2 in lung cancer is predictive of poor survival

doi: 10.1186/s12885-015-1658-2

Figure Lengend Snippet: AGR2 staining intensity of lung tissue. Staining is scored by integrated intensity values on the vertical axis for the tissue types listed on the horizontal axis. Normal bronchial epithelium shows the strongest staining for AGR2. The number of tissue examples scored is indicated by n

Article Snippet: AGR2 expression was evaluated on the TMA with NB110-17780 rabbit polyclonal (Novus Biologicals) at 2 μg/ml concentration as described [ ].

Techniques: Staining

Journal: BMC Cancer

Article Title: High expression of AGR2 in lung cancer is predictive of poor survival

doi: 10.1186/s12885-015-1658-2

Figure Lengend Snippet: AGR2 expression and tumor grade. The box plots show a decrease in integrated intensity values with increasing tumor grades (top panel). This correlation was not evident when separated by tumor types (middle panel for adenocarcinoma; bottom panel for squamous carcinoma). Adenocarcinoma grade 3 shows low staining intensity but squamous carcinoma shows higher staining intensity

Article Snippet: AGR2 expression was evaluated on the TMA with NB110-17780 rabbit polyclonal (Novus Biologicals) at 2 μg/ml concentration as described [ ].

Techniques: Expressing, Staining

Journal: BMC Cancer

Article Title: High expression of AGR2 in lung cancer is predictive of poor survival

doi: 10.1186/s12885-015-1658-2

Figure Lengend Snippet: AGR2 expression in primary vs . metastatic sites. No significant differences in AGR2 expression was seen in primary, lymph node metastasis, and distant metastasis. This applied to adenocarcinomas, squamous carcinomas and large cell carcinomas

Article Snippet: AGR2 expression was evaluated on the TMA with NB110-17780 rabbit polyclonal (Novus Biologicals) at 2 μg/ml concentration as described [ ].

Techniques: Expressing

Journal: BMC Cancer

Article Title: High expression of AGR2 in lung cancer is predictive of poor survival

doi: 10.1186/s12885-015-1658-2

Figure Lengend Snippet: AGR2 immunostaining of lung epithelial cells. Shown are examples of lung epithelial cells stained for AGR2: a , normal bronchiolar epithelium; b , bronchioloalveolar carcinoma, mucinous type; c , moderately differentiated adenocarcinoma; d , moderately differentiated squamous carcinoma; e , poorly differentiated adenocarcinoma; f , large cell carcinoma

Article Snippet: AGR2 expression was evaluated on the TMA with NB110-17780 rabbit polyclonal (Novus Biologicals) at 2 μg/ml concentration as described [ ].

Techniques: Immunostaining, Staining

Journal: BMC Cancer

Article Title: High expression of AGR2 in lung cancer is predictive of poor survival

doi: 10.1186/s12885-015-1658-2

Figure Lengend Snippet: High vs . low tumor AGR2 expression. The percentages of tissue cores showing low vs . high integrated intensity values for the different tumor grades in adenocarcinoma and squamous carcinoma are tabulated

Article Snippet: AGR2 expression was evaluated on the TMA with NB110-17780 rabbit polyclonal (Novus Biologicals) at 2 μg/ml concentration as described [ ].

Techniques: Expressing

Journal: BMC Cancer

Article Title: High expression of AGR2 in lung cancer is predictive of poor survival

doi: 10.1186/s12885-015-1658-2

Figure Lengend Snippet: High vs . low tumor AGR2 expression. The percentages of tissue cores showing low vs . high integrated intensity values for the different tumor grades in adenocarcinoma and squamous carcinoma are tabulated

Article Snippet: AGR2 expression was evaluated on the TMA with NB110-17780 rabbit polyclonal (Novus Biologicals) at 2 μg/ml concentration as described [ ].

Techniques: Expressing

Journal: BMC Cancer

Article Title: High expression of AGR2 in lung cancer is predictive of poor survival

doi: 10.1186/s12885-015-1658-2

Figure Lengend Snippet: AGR2 expression and smoking status. Non-smokers had on average slightly higher levels than smokers

Article Snippet: AGR2 expression was evaluated on the TMA with NB110-17780 rabbit polyclonal (Novus Biologicals) at 2 μg/ml concentration as described [ ].

Techniques: Expressing

Journal: BMC Cancer

Article Title: High expression of AGR2 in lung cancer is predictive of poor survival

doi: 10.1186/s12885-015-1658-2

Figure Lengend Snippet: Kaplan-Meier survival plot for AGR2 expression levels. For patients under 65, high tumor AGR2 expression is correlated with a poorer survival than low tumor AGR2 expression

Article Snippet: AGR2 expression was evaluated on the TMA with NB110-17780 rabbit polyclonal (Novus Biologicals) at 2 μg/ml concentration as described [ ].

Techniques: Expressing

Journal: BMC Cancer

Article Title: High expression of AGR2 in lung cancer is predictive of poor survival

doi: 10.1186/s12885-015-1658-2

Figure Lengend Snippet: Multivariate cox model for younger patients including AGR2, tumor stage, grade and patient age

Article Snippet: AGR2 expression was evaluated on the TMA with NB110-17780 rabbit polyclonal (Novus Biologicals) at 2 μg/ml concentration as described [ ].

Techniques:

Journal: Journal of Translational Medicine

Article Title: SATB1 is overexpressed in metastatic prostate cancer and promotes prostate cancer cell growth and invasion

doi: 10.1186/1479-5876-11-111

Figure Lengend Snippet: SATB1 immunohistochemistry staining. A , benign prostate hyperplasia. B , prostatic carcinoma with metastasis. C , prostatic carcinoma without metastasis. D , negative control. Magnification: 400 × .

Article Snippet: The sections were blocked with 2% goat serum in 0.01 M PBS at room temperature for 1 h, then incubated with rabbit polyclonal anti-SATB1 antibody (1:500 dilution, NB110-17780, Novus Biologicals, Littleton, CO, USA) overnight at 4°C.

Techniques: Immunohistochemistry, Staining, Negative Control

Journal: Journal of Translational Medicine

Article Title: SATB1 is overexpressed in metastatic prostate cancer and promotes prostate cancer cell growth and invasion

doi: 10.1186/1479-5876-11-111

Figure Lengend Snippet: SATB1 expression and clinicopathologic parameters of prostate cancer

Article Snippet: The sections were blocked with 2% goat serum in 0.01 M PBS at room temperature for 1 h, then incubated with rabbit polyclonal anti-SATB1 antibody (1:500 dilution, NB110-17780, Novus Biologicals, Littleton, CO, USA) overnight at 4°C.

Techniques: Expressing

Journal: Journal of Translational Medicine

Article Title: SATB1 is overexpressed in metastatic prostate cancer and promotes prostate cancer cell growth and invasion

doi: 10.1186/1479-5876-11-111

Figure Lengend Snippet: SATB1 expression is correlated with the invasion ability of prostate cancer cells. A - C : The invasion of LNCaP, DU-145 and PC-3 cells was measured by Transwell chamber. A , DU-145. B , PC-3. C , LNCaP. Magnification: 200×. D : Western blot analysis of the relative protein level of SATB1 in LNCaP, DU-145 and PC-3 cells (n = 3). β-actin served as loading control. *P < 0.05 compared to DU-145 cells. Note that SATB1 antibody could not detect SATB2 protein in the cell lysates.

Article Snippet: The sections were blocked with 2% goat serum in 0.01 M PBS at room temperature for 1 h, then incubated with rabbit polyclonal anti-SATB1 antibody (1:500 dilution, NB110-17780, Novus Biologicals, Littleton, CO, USA) overnight at 4°C.

Techniques: Expressing, Western Blot, Control

Journal: Journal of Translational Medicine

Article Title: SATB1 is overexpressed in metastatic prostate cancer and promotes prostate cancer cell growth and invasion

doi: 10.1186/1479-5876-11-111

Figure Lengend Snippet: Silencing of SATB1 in DU-145 cells. DU-145 cells were untransfected (control), transfected with pSliencer3.1 control vector or transfected with pSilencer3.1-SATB1 vector for 24 h, 48 h, or 72 h. The protein levels of SATB1 and SATB2 were detected by Western blot analysis (n = 3). β-actin served as loading control. * P < 0.05 compared to cells transfected with pSliencer3.1.

Article Snippet: The sections were blocked with 2% goat serum in 0.01 M PBS at room temperature for 1 h, then incubated with rabbit polyclonal anti-SATB1 antibody (1:500 dilution, NB110-17780, Novus Biologicals, Littleton, CO, USA) overnight at 4°C.

Techniques: Control, Transfection, Plasmid Preparation, Western Blot

Journal: Journal of Translational Medicine

Article Title: SATB1 is overexpressed in metastatic prostate cancer and promotes prostate cancer cell growth and invasion

doi: 10.1186/1479-5876-11-111

Figure Lengend Snippet: Silencing of SATB1 inhibits the invasion and growth of DU-145 cells. A - D : The invasion of DU-145 cells was measured by Transwell chamber. A, DU-145 cells untransfected. B , DU-145 cells transfected with pSliencer3.1. C , DU-145 cells transfected with pSilencer3.1-SATB1. Magnification: 200×. D : Quantitative analysis of the number of invaded cells as shown in A - C (n = 3). E : The proliferation of DU-145 cells was determined by CCK8 assay (n = 3). The cell proliferation inhibition rate was calculated for DU-145 cells at 24 h, 48 h and 72 h after transfection with pSilencer3.1-SATB1. *P < 0.05 compared to cells transfected with pSliencer3.1.

Article Snippet: The sections were blocked with 2% goat serum in 0.01 M PBS at room temperature for 1 h, then incubated with rabbit polyclonal anti-SATB1 antibody (1:500 dilution, NB110-17780, Novus Biologicals, Littleton, CO, USA) overnight at 4°C.

Techniques: Transfection, CCK-8 Assay, Inhibition

Journal: Journal of Translational Medicine

Article Title: SATB1 is overexpressed in metastatic prostate cancer and promotes prostate cancer cell growth and invasion

doi: 10.1186/1479-5876-11-111

Figure Lengend Snippet: Reconstitution of SATB1 expression in LNCaP cells. LNCaP cells were untransfected (control), transfected with pcDNA3.1 control vector or transfected with pcDNA3.1-SATB1 vector for 12 h, 24 h, or 36 h. A . The mRNA level of SATB1 was detected by RT-PCR (n = 3). B . The protein level of SATB1 was detected by Western blot analysis (n = 3). β-actin served as loading control. *P < 0.05 compared to cells transfected with pcDNA3.1.

Article Snippet: The sections were blocked with 2% goat serum in 0.01 M PBS at room temperature for 1 h, then incubated with rabbit polyclonal anti-SATB1 antibody (1:500 dilution, NB110-17780, Novus Biologicals, Littleton, CO, USA) overnight at 4°C.

Techniques: Expressing, Control, Transfection, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: Journal of Translational Medicine

Article Title: SATB1 is overexpressed in metastatic prostate cancer and promotes prostate cancer cell growth and invasion

doi: 10.1186/1479-5876-11-111

Figure Lengend Snippet: Overexpression of SATB1 promotes the invasion and growth of LNCaP cells. A - D : The invasion of LNCaP cells was measured by Transwell chamber. A , LNCaP cells untransfected. B , LNCaP cells transfected with pcDNA3.1. C , LNCaP cells transfected with pcDNA3.1-SATB1. Magnification: 200×. D : Quantitative analysis of the number of invaded cells as shown in A - C (n = 3). *P < 0.05 compared to cells transfected with pcDNA3.1. E : The proliferation of LNCaP cells was determined by CCK8 assay (n = 3). The cell proliferation ratio was calculated for LNCaP cells at 24 h, 48 h and 72 h after transfection of pcDNA3.1 or pcDNA3.1-SATB1. *P < 0.05 compared to corresponding cells transfected with pcDNA3.1.

Article Snippet: The sections were blocked with 2% goat serum in 0.01 M PBS at room temperature for 1 h, then incubated with rabbit polyclonal anti-SATB1 antibody (1:500 dilution, NB110-17780, Novus Biologicals, Littleton, CO, USA) overnight at 4°C.

Techniques: Over Expression, Transfection, CCK-8 Assay